Biochemical Profile and Genetic Polymorphism of MTHFRC677T in Risk of Type 2 Diabetes Mellituss
Mushtaq Ahmad Bhat,
Showkat Ahmad Bhat,
Sheikh Bilal Ahmad,
Wasim Qureshi,
Sabhiya Majid,
Aarif Ali,
Ishraq Hussain,
Tehseen Hassan,
Muneeb U. Rehman,
Manzoor R. Mir
Issue:
Volume 2, Issue 2, June 2017
Pages:
19-25
Received:
26 December 2016
Accepted:
17 March 2017
Published:
11 April 2017
DOI:
10.11648/j.ijde.20170202.11
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Abstract: Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15±0.39 p>0.05) and (1.19±0.70 vs 1.01±0.52 p>0.060) respectively. Low density lipoprotein (LDL) and total cholesterol (TC) are significantly higher in patients than control group (4.09±1.14 vs 3.01±1.02 p<0.0002) and (4.21±1.28 vs 3.78±1.29 p<0.05). However, HDL/TC ratio is significantly higher in patients than controls (0.21±0.91 vs 0.30±0.99 p<0.05). Serum levels of all liver enzymes (ALT, AST, and ALP) analyzed are significantly higher in patients than controls (12.69±10.80 vs 4.95±2.66, p<0.0002), (15.99±10.70 vs 6.95±3.84, p<0.0002) and 68.29±27.78 vs 21.27±7.77, p<0.0001) respectively. On genetic level the role of MTHFR C677T polymorphisms our results showed 63% of the cases showed homozygous mutant condition. The allelic association of polymorphism of controls with cases was found to be significant (P=0.007). Homozygous mutant condition of MTHFRC677T gene was found to be certainly higher in Diabetes Mellitus 2 Cases of above 60 years of age (80%), than ages below 60 years and in controls (16.6%) and was significant as p=0.005, compared to below 60 years of age (33.3%) and in controls (0%) and association was insignificant as p=0.4667. Our data suggest that there is an important role of LDL, TC, HDL/TC, ALT, AST, and ALP in type-2 Diabetes, also gene polymorphisms of MTHFR C677T gene may act synergistically to increase the risk of type 2 diabetes.
Abstract: Diabetes mellitus (DM) is a common endocrine metabolic disorder and a leading cause of death worldwide Diabetes type-2 is a multicausal disease which develops slowly and in a stepwise order. Our study showed there was no significant difference in serum high density lipoprotein (HDL) and Tri glyceride (TG) of patients and controls (0.90±0.59 vs 1.15...
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Genetic Polymorphism and Personalized Medicine-Application in Metabolic Syndrome
Ri-Hyang Paek,
Dae-Yong Jon,
Hak-Chol Ri,
Yong-Ju Gong
Issue:
Volume 2, Issue 2, June 2017
Pages:
26-29
Received:
24 February 2017
Accepted:
1 April 2017
Published:
18 May 2017
DOI:
10.11648/j.ijde.20170202.12
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Abstract: To explore the associations between the phenotypes of metabolic syndrome and several genetic polymorphisms in the people of the Democratic People’s Republic of Korea, and to address the basic and practical problems that arise in carrying out the personalized medicine of metabolic syndrome. We analyzed the four phenotypes of metabolic syndrome including hypertension, dyslipidemia, diabetes mellitus and obesity by PCR. And we could select six genetic polymorphisms that are associated with more than three phenotypes (3 of 4 phenotypes including hypertension, dyslipidemia, diabetes mellitus and obesity) of metabolic syndrome; they are CMA (Chymase) A (-1903)G, GNB3(β3 subunit of G protein) C825T and C1429T, eNOS (Nitric oxide synthetase in the endothelium) 4a/4b and G894T, and MTHFR (Methylenetetra hydrofolate reductase) C677T. It shows that these can be the significant markers related with metabolic syndrome in the future. (It recommends future studies to support our conclusion) The typical genes associated with metabolic syndrome in the people of the Democratic People’s Republic of Korea will be basic data for the personalized medicine in metabolic syndrome.
Abstract: To explore the associations between the phenotypes of metabolic syndrome and several genetic polymorphisms in the people of the Democratic People’s Republic of Korea, and to address the basic and practical problems that arise in carrying out the personalized medicine of metabolic syndrome. We analyzed the four phenotypes of metabolic syndrome inclu...
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Preventive Effect of Phloretin on Components of Glycoprotein Changes in Streptozotocin Induced Diabetic Rats
Tamilarasan Nithiya,
Rajangam Udayakumar
Issue:
Volume 2, Issue 2, June 2017
Pages:
30-35
Received:
28 February 2017
Accepted:
28 April 2017
Published:
3 July 2017
DOI:
10.11648/j.ijde.20170202.13
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Abstract: The present study was aimed to evaluate the protective effect of phloretin on glycoprotein components in serum and tissues of streptozotocin induced diabetic rats. Diabetes was induced in male Wistar rats by a single intraperitoneal injection of STZ (60 mg/kg b.w). Phloretin (25mg and 50mg/kg b.w) was administered orally to diabetic rats for 45 days. The effect of phloretin on serum glucose, glycated hemoglobin, serum and tissue glycoprotein components were studied. Phloretin administration to diabetic rats decreased the level of glucose, glycated hemoglobin and glycoprotein components in serum. There was observed a significant decrease in the level of sialic acid and significantly elevated levels of hexose, hexosamine and fucose in liver and kidney of diabetic rats. The altered levels of serum and tissue glycoprotein components were restored to near normal in diabetic rats treated with phloretin at 50mg/kg b.w. The present findings suggest that phloretin can potentially ameliorates the hyperglycemia and changes in glycoprotein components abnormalities in streptozotocin induced diabetic rats. So, the phloretin may be used as an effective therapeutic agent for diabetes mellitus in future.
Abstract: The present study was aimed to evaluate the protective effect of phloretin on glycoprotein components in serum and tissues of streptozotocin induced diabetic rats. Diabetes was induced in male Wistar rats by a single intraperitoneal injection of STZ (60 mg/kg b.w). Phloretin (25mg and 50mg/kg b.w) was administered orally to diabetic rats for 45 day...
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